Fig. 4. Effects of Mcl-1 knockdown and pan-caspase inhibitor on activation of caspase-3 in MeT-5A and MSTO-211H cells. Cells were transfected with 10 nM Mcl-1-targeting siRNA (siMcl-1) or Stealth RNAi control (siC) for 24 h, after which cells were incubated with resveratrol (15 μM) and clofarabine (40 nM) for 48 h in the presence or absence of Z-VAD (10 μM). (A) The percentage of apoptotic cells after annexin V binding was analyzed by a Muse cell analyzer. (B) Caspase-3/7 activity was measured by ApoToxGloTM Triplex Assay. Error bars represent the mean ± SEM for three independent experiments. Cl/R, clofarabine plus resveratrol. *P ＜ 0.05 compared to respective controls.
