Fig. 4.

Effect of high-dose irradiation on expression of miR-545 and Ku70 in Lewis lung cancer cells. 24 h after irradiated with a single dose of 5 Gy/1f/1d, cells were lyzed to detect expression of miR-545 (a) and Ku70 mRNA by using quantity RT-PCR (b) and Ku protein by western blot (c). The cells were transfected with miR-545 inhibitor and then exposed to radiation followed by detection on expression of Ku70 mRNA (d) and Ku70 protein (e). The results were represented as mean ± SD. *P < 0.05 compared with corresponding control