Fig. 2.

Dose-dependent indirect hemolytic activity of VRV-PL-VIIIa. VRV-PL-VIIIa (0–5 μg) in 100 μL of phosphate-buffered saline (PBS) was incubated with erythrocytes, egg yolk and PBS (1:1:8 v/v) for 10 min at 37 °C. The released hemoglobin in the supernatant was measured by reading absorbance at 540 nm. The results are expressed as ± S.E.M. (n = 4)