Upstream movement of pre-spawn female (a) and male (b) sea lamprey. Squares, trials where 3kPZS was administered to the stream (dashed regression line, females R2=0.59, males R2=0.38); upward triangles, trials where 50% methanol (control) was administered to the stream (solid regression line, females R2=0.68, males R2=0.57); and filled circles, trials where larval extract was administered to the stream (dotted-dashed regression line, females R2=0.10, males R2=0.85). q1–q3=the lower, median and upper quartiles of temperatures. See Results and table 2, for statistical comparisons at each quartile of temperature.