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. 2015 Jun 4;15:48. doi: 10.1186/s12896-015-0168-2

Table 1.

Oligonucleotide primers used for multiplex PCR amplification

Primer set Source Sequence Product size (bp)
lacZB Bej et al., 1990 F: 5′ ATGAAAGCTGGCTACAGGAAGGCC 3′ 876
R: 5′ CACCATGCCGTGGGTTTCAATATT 3′
lacZ3 This work F: 5′ TTGAAAATGGTCTGCTGCTG 3′ 234
R: 5′ TATTGGCTTCATCCACCACA 3′
uidA Bej et al., 1991 F: 5′ TGGTAATTACCGACGAAAACGGC 3′ 162
R: 5′ ACGCGTGGTTACAGTCTTGCG 3′
yaiO This work F: 5′ TGATTTCCGTGCGTCTGAATG 3′ 115
R: 5′ ATGCTGCCGTAGCGTGTTTC 3′