Table 2.

Comparison of expected uidA and yaiO PCR products by Primer-BLAST analysis

Organism	Total number of hits		yaiO vs. uidA
	yaiO +	uidA +	yaiO + uidA -	yaiO + uidA+	yaiO – uidA +
Escherichia coli	83	91	31	52	39
Escherichia sp.	1	2	1		2
Citrobacter rodentium		1			1
Shigella boydii	1	2		1	1
Shigella flexneri		6			6
Shigella sonnei	2	2		2
	87	104

The number of potential target sequences yielding positive in silico PCR amplification (N = 136) with yaiO or uidA primers (Table 1) are grouped by species or genus. The genomes that produce single and double amplicons are compared on the right