Figure 6.

Activation of CB₁ does not enhance proliferation of bCECs. (A) In a proliferation assay, CP55940 (100 nM) and WIN55212 (100 nM) do not enhance bCEC proliferation. (B) A concentration-response profile for CP55940 again shows no effect on proliferation, while EGF (50 ng/mL) enhances proliferation. (C) Epidermal growth factor enhances proliferation in a concentration-dependent manner in our preparation. (D) CP55940 (100 nM) in combination with half-maximal (5 ng/mL) or low (1 ng/mL) EGF does not enhance proliferation. (B) *P < 0.05 1-way ANOVA/Dunnett's post hoc versus control. (D) Not significant (NS), 1-way ANOVA/Bonferroni post hoc between EGF 5 ng/mL and EGF 5 ng/mL + CP (100 nM).