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. 2014 Dec 16;112(2):329–337. doi: 10.1038/bjc.2014.589

Figure 5.

Figure 5

LBH589 inhibits differentiation by ATRA and reduces BCL-xL. (A, B) Lysates were made from NB4 cells treated as noted in Figure 4A. Immunoblot was used to detect the protein expression of C/EBPɛ, BCL-xL, BCL-2, active/cleaved caspase-3, and HDAC1 as a loading control. (B) All samples were analysed on one membrane. (C) The results of FACS analyses are shown. NB4 cells were treated as stated in Figure 4A. Cells were stained for CD11b. (D) Immunoblot analysis of NB4 whole-cell protein lysates was used to assess the levels of PML-RARα. NB4 cells were treated with 100 nM LBH589 (LBH) for 24 h. (E) Lysates were made from NB4 cells treated as noted in Figure 4B. Immunoblot was used to detect the protein expression of BCL-2, BCL-xL, and HDAC1 as a loading control. Lysates treated with VPA and LBH589 were spotted onto one membrane for direct comparison.