Table 2. Pimonidazole gene signature.

			Pimonidazole		Ki67
ProbeIDa	Gene symbolb	Gene nameb	Corrc	P-valuec	Corrc	P-valuec
Proliferation gene setsd
2630673	ASF1B	Antisilencing function 1B histone chaperone	0.39	0.013	0.29	0.052
6130441	ASPM	Abnormal spindle homolog, microcephaly associated (Drosophila)	0.39	0.016	0.57	<0.001
1230682	BIRC5	Baculoviral IAP repeat containing 5	0.36	0.023	0.33	0.026
6350348	BUB3	BUB3 mitotic checkpoint protein	0.35	0.029	0.29	0.048
2510639	CENPE	Centromere protein E, 312 kDa	0.45	0.004	0.26	0.078
2120678	CENPU	Centromere protein U	0.33	0.043	0.17	0.27
6020746	CMTM3	CKLF-like MARVEL transmembrane domain containing 3	0.49	0.002	0.20	0.18
990725	DONSON	Downstream neighbor of SON	0.33	0.039	0.41	0.005
5570296	DTL	Denticleless E3 ubiquitin protein ligase homolog (Drosophila)	0.42	0.008	-0.07	0.65
540053	FOXM1	Forkhead box M1	0.39	0.014	0.31	0.034
6520095	G6PD	Glucose-6-phosphate dehydrogenase	0.38	0.018	-0.10	0.49
3180367	HJURP	Holliday junction recognition protein	0.38	0.018	0.54	<0.001
6770408	MCM2	Minichromosome maintenance complex component 2	0.52	0.001	0.42	0.003
1820176	MEP1A	Meprin A, alpha (PABA peptide hydrolase)	0.32	0.047	0.28	0.058
3190768	MTMR2	Myotubularin-related protein 2	0.32	0.049	-0.12	0.42
5700373	TRIP13	Thyroid hormone receptor interactor 13	0.34	0.037	0.54	<0.001
650156	ZWINT	ZW10-interacting kinetochore protein	0.33	0.042	−0.03	0.85
DNA repair gene setsd
1990253	TDG	Thymine-DNA glycosylase	0.39	0.014	0.38	0.009
3610750	UNG	Uracil-DNA glycosylase	0.36	0.024	0.32	0.033
430100	XRCC6	X-ray repair complementing defective repair in Chinese hamster cells 6	0.40	0.011	0.43	0.003
Hypoxia gene setd
5670465	ADM	Adrenomedullin	0.36	0.023	0.06	0.69
3190148	DDIT4	DNA-damage-inducible transcript 4	0.44	0.005	0.34	0.02
3610672	DSP	Desmoplakin	0.35	0.03	0.23	0.13
4640039	FER1L4	Fer-1-like 4 (C. elegans), pseudogene	0.42	0.008	0.05	0.72
7320441	HILPDA	Hypoxia-inducible lipid droplet-associated	0.37	0.021	0.03	0.87
4220731	P4HA1	Prolyl 4-hydroxylase, alpha polypeptide I	0.38	0.018	0.35	0.017
1820424	PGAM4	Phosphoglycerate mutase family member 4	0.36	0.024	0.05	0.73
2030093	PKMe	Pyruvate kinase, muscle	0.42	0.009	0.22	0.14
6840619	RIMKLA	Ribosomal modification protein rimK-like family member A	0.39	0.014	0.40	0.006
3930372	RNASE4	Ribonuclease, RNase A family, 4	0.46	0.004	0.10	0.50
2140128	SCD	Stearoyl-CoA desaturase (delta-9-desaturase)	0.37	0.022	0.04	0.77
1190670	SPAG4	Sperm-associated antigen 4	0.32	0.047	0.14	0.37

Abbreviations: AR=androgen receptor; Corr=correlation coefficient; HIF1=hypoxia-inducible factor 1.

^aIllumina probe ID.

^bHUGO gene symbol and gene name.

^cCorr and P-value in Spearman's correlation analysis of pimonidazole immunoscore or Ki67 labelling index vs gene expression.

^dGenes in bold are known direct HIF1 targets, and underlined genes are known direct AR targets.

^eAlso present in the proliferation gene sets.