Table 3.

Effect of temperature on stability of TaGH2 and TbGH2.

	3 h		24 h
	TaGH2	TbGH2	TaGH2	TbGH2
50°C	127 ± 5	129 ± 9	151 ± 4	246 ± 12
60°C	123 ± 2	199 ± 7	108 ± 8	202 ± 10
70°C	123 ± 4	94 ± 3	47 ± 3	0
80°C	128 ± 2	17 ± 6	15 ± 3	0
90°C	127 ± 3	0	0	0

Activity of TaGH2 and TbGH2 was measured at 90°C in 20 mM citrate or maleate buffer (pH 6.5) with 2 mM 4-Np-β-D-glucopyranoside for 10 min after preincubation for 3 or 24 h, respectively, at different temperatures (50–90°C). Activities determined at 0 h were set to 100%.