Table 4.

Effects of metal ions on the activity of TaGH2 and TbGH2.

Reagent	Relative activity of TaGH2 (%)			Relative activity of TbGH2 (%)
	1 mM	5 mM	10 mM	1 mM	5 mM	10 mM
None	100 ± 3	100 ± 5	100 ± 2	100 ± 4	100 ± 4	100 ± 5
AgNO3	0	0	0	0	0	0
AlCl3	97 ± 4	53 ± 1	17 ± 5	149 ± 1	98 ± 13	0
CaCl2	100 ± 3	100 ± 4	103 ± 4	93 ± 6	72 ± 5	56 ± 20
CoCl2	99 ± 3	101 ± 2	97 ± 2	52 ± 7	0	0
CrCl3	88 ± 2	43 ± 3	13 ± 5	139 ± 4	53 ± 11	0
CuCl2	98 ± 4	64 ± 5	20 ± 4	0	0	0
FeCl2	97 ± 1	42 ± 3	0	117 ± 3	31 ± 14	0
FeCl3	95 ± 2	53 ± 4	12 ± 3	120 ± 2	37 ± 18	0
KCl	99 ± 3	98 ± 3	102 ± 4	146 ± 4	144 ± 7	83 ± 5
MgCl2	99 ± 1	103 ± 2	103 ± 2	136 ± 4	117 ± 2	113 ± 7
MnCl2	96 ± 3	98 ± 1	88 ± 4	94 ± 15	86 ± 3	64 ± 12
NaCl	97 ± 2	99 ± 4	100 ± 4	138 ± 11	129 ± 6	104 ± 10
NiCl2	99 ± 3	104 ± 4	90 ± 3	61 ± 13	0	0
RbCl	96 ± 2	102 ± 1	98 ± 1	143 ± 10	143 ± 2	81 ± 5
SrCl2	101 ± 3	104 ± 4	104 ± 2	127 ± 4	106 ± 9	67 ± 15
ZnCl2	99 ± 2	97 ± 2	64 ± 3	0	0	0

Activity of TaGH2 and TbGH2 was measured at 90°C in 20 mM citrate or maleate buffer (pH 6.5) with 2 mM 4-Np-β-D-glucopyranoside for 10 min in presence or absence of metal ions.