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. 2015 Mar 17;6(2):e02486-14. doi: 10.1128/mBio.02486-14

FIG 6 .

FIG 6 

G3BP1 and Caprin1 regulate PKR recruitment to antiviral SGs. (A) Western blots for Caprin1, G3BP1, and GAPDH were performed on control, G3BP1, or Caprin1 siRNA-treated HeLa cells, as indicated. (B) PKR localization was detected by IF in HeLa cells treated with siControl, siG3BP1, and siCaprin1 and infected for 14 h with Mengo-Zn. PKR intensity was normalized to Tia1 intensity for each granule and presented as a relative value. (C) HeLa cells treated with control (Con), G3BP1, or Caprin1 (Cap1) siRNAs and infected as described for panel B were stained and quantified for P-eIF2α intensity and are presented in a box and whisker plot. (D) HeLa cells were treated with either control or Caprin1 (Cap1)-specific siRNAs and then transfected with GFP-tagged G3BP1 (green) to induce P-PKR. Cells were fixed and stained for P-eIF2α (red) and DAPI (blue). (E) Levels of P-eIF2α were quantified from panel D as previously described (6). All plots with statistics were analyzed with a Student t test (**, P < 0.01; ***, P < 0.001).