FIG 1 .

Relevance of the poly(A) tail in the binding of the 3′-end-interacting cellular proteins. Proteins (600 µg) from cytoplasmic extracts of TGEV-infected Huh-7 cells were pulled down without RNA (−) or with TGEV genome ends 5′ (5′) and 3′ without (3′) and with (3′ A) poly(A) tail, separated in SDS-PAGE gels, and analyzed by immunoblotting with specific antibodies against PABP, EPRS, RRS, p100, and the hnRNPs (Q, A1, U, and A0).