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. 2015 Mar 10;6(2):e00105-15. doi: 10.1128/mBio.00105-15

FIG 4 .

FIG 4 

Analysis of the interaction of EPRS and RRS with viral RNAs by RNA immunoprecipitation assay. Cytoplasmic extracts of TGEV-infected ST cells were immunoprecipitated with specific antibodies for EPRS (α-EPRS), RRS (α-RRS), and GFP (α-GFP). The presence of viral genomic RNA (gRNA) and sgmRNAs (S, 3a, M, E, N, and 7 genes) in the immunoprecipitated RNA-protein complexes was quantified by RT-qPCR and expressed as the fold change of the RNA levels relative to the RNA levels in the samples immunoprecipitated with GFP antibody. Error bars represent the standard deviations from three independent experiments.