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. 2015 Mar 10;6(2):e00105-15. doi: 10.1128/mBio.00105-15

FIG 5 .

FIG 5 

Study of the interaction between EPRS and the TGEV GAIT-like RNA motif by RNA EMSA. Purified EPRS, DDK/Myc tag, and GAPDH proteins were incubated with the 3′-biotinylated GAIT-like RNA motif riboprobe (GAIT-like). The RNA-protein complexes were resolved by electrophoresis on a nondenaturing 4% polyacrylamide gel, and the biotinylated riboprobe was detected using the BrightStar BioDetect kit (Ambion). The arrow indicates the shifted protein-RNA complex.