FIG 6 .

In vitro analysis of the inhibitory effect on translation of the TGEV GAIT-like RNA motif. Three chimeric luciferase reporter mRNA constructs, containing at the 3′ UTR no GAIT element (No GAIT), the Cp GAIT element (Cp GAIT), or the TGEV GAIT-like RNA motif (TGEV GAIT), were subjected to in vitro translation in a rabbit reticulocyte lysate in the absence (blue bars) or in the presence (red bars) of the GAIT complex. The translation of these chimeric constructs was quantitated by chemiluminescence and expressed as the percentage of translation of each construct in the presence of the GAIT complex compared to that in the absence of the GAIT complex. Error bars represent the standard deviations from three independent experiments. ***, P < 0.001.