TABLE 1 .
Oligonucleotides used for PCR amplifications
| Oligonucleotidea | Oligonucleotide sequence (5′→3′)b |
|---|---|
| XmaI-sp6-5′-VS | CGCGCCCGGGATTTAGGTGACACTATA(G)ACTTTTAAAGTAAAGTGAGTGTAGC |
| 5′TGEV-504-RS | CACCAATGACGTAGTGATCCTTACG |
| XmaI-T7-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGATGCTGTATTTATTACAGTTTTAATC |
| TGEV-3′-RS | GTATCACTATCAAAAGGAAAATTTTCAAACAATTTTAC |
| TGEV-3′-pA-RS | TTTTTTTTTTTTTTTTTTTTTTTTGTATCACTATCAAAAGG |
| F1-RS | CTATATAAGATATTAAAGTCATTGACAGTTTTAAGATTG |
| F2-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGACTATTACTTAATCACTCTTTCAAT |
| F2-RS | GCAAAATCCTAGTAAGACTAGAAAGATTACTCGAAGC |
| F3-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGATTTTGCTGCTACAGATTGTTAG |
| F3-RS | GTGCTTACCATTCTGTACAAGAGTAGACAGCG |
| F4-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGCTGTCTACTCTTGTACAGAATGGTA |
| F4-RS | CTTTAAACTTCTCTAAATTACTAAATCTAGCATTGC |
| F5-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGTTAGTCACATTAATGTAAGGCAAC |
| F5-RS | CTAATATGCAATAGGGTTGCTTGTACCTCCTATTAC |
| F6-VS | CGCGCCCGGGTAATACGACTCACTATA(G)GGAGAAGTTTAAAGATCCGCTACGA |
a
VS, virus sense; RS, reverse sense.
b
Transcription promoters are in bold. Transcription initiation sites are in parentheses. The template sequence corresponding to poly(A) is underlined