TABLE 3 .
Amplicon | Oligonucleotidea | Oligonucleotide sequence (5′→3′)b | Methodc |
---|---|---|---|
gRNA | Rep-VS | TTCTTTTGACAAAACATACGGTGAA | SYBR |
Rep-RS | CTAGGCAACTGGTTTGTAACATCTTT | SYBR | |
sgmRNA S | LCS1-VS | CCAACTCGAACTAAACTTTGGTAACC | SYBR |
LCS1-RS | TCAATGGCATTACGACCAAAAC | SYBR | |
sgmRNA 3a | rt3a-VS2 | CGGACACCAACTCGAACTAAACTTAC | SYBR |
rt3a-RS | ATCAAGTTCGTCAAGTACAGCATCTAC | SYBR | |
sgmRNA E | Ldrt-VS | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | SYBR |
mRNAE-RS | CAATGCCCTAGGAAACGTCATAG | SYBR | |
sgmRNA M | Ldrt-VS | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | SYBR |
mRNAM-RS | GCATGCAATCACACACGCTAA | SYBR | |
sgmRNA N | Ldrt-VS | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | SYBR |
N82−RS | TCTTCCGACCACGGGAATT | SYBR | |
sgmRNA 7 | Ldrt-VS | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | SYBR |
7 (38)-RS | AAAACTGTAATAAATACAGCATGGAGGAA | SYBR | |
gRNA | Rep-VS | TTCTTTTGACAAAACATACGGTGAA | TaqMan |
Rep-RS | CTAGGCAACTGGTTTGTAACATCTTT | TaqMan | |
Rep-MGB | FAM-AGGGCACCGTTGTCA-MGB | TaqMan | |
sgmRNA 7 | Ldrt-VS | CGTGGCTATATCTCTTCTTTTACTTTAACTAG | TaqMan |
7 (38)-RS | AAAACTGTAATAAATACAGCATGGAGGAA | TaqMan | |
mRNA7-MGB | FAM-CGAACTAAACGAGATGCT-MGB | TaqMan |
VS, virus sense; RS, reverse sense; MGB, minor groove binder group.
FAM, 6-carboxyfluorescein.
SYBR, SYBR green-based detection chemistry for qPCR; TaqMan, TaqMan-based detection for qPCR.