FIG 1.

Effect of multiplicity of infection on PRV 180 capsid motility and infection efficiency during axonal infection. (A) The trichamber neuron culture is schematically represented at the top of the figure (soma [S], middle [M], and neurite [N] compartments). Low-magnification images of cell bodies in the S compartment and high-magnification images of axons in the N compartment are illustrated. (B) PRV 180 capsids (red fluorescent puncta) in infections with different MOIs (MOIs of 100, 10, and 1) in N-compartment axons are shown (0.5 to 1 h postinfection [hpi]). (a, c, and e) Capsid puncta (bars = 10 µm); (b, d, and f) merged images of capsid puncta and axons. In panels e and f, the white arrowhead points to a single capsid puncta. (C) Maximum-intensity projection of the video frames in Movie S1 in the supplemental material. Movement is recorded as lines of puncta. The black arrowhead in the projection for 1 MOI points to the short line corresponding to the back-and-forth movement of a single capsid (bars = 20 µm). (D) PRV 180 retrograde infection at an MOI of 1 in trichambers. Low-magnification images of cell bodies in the S compartment are shown. Virus inoculum was added to the N-compartment axons. The lipophilic dye DiO was added to the N-compartment medium at 3 hpi. Images of S compartments were taken at 24 and 48 hpi. Three independent experiments were performed. Bars = 1,000 µm.