FIG 2.

High-MOI UV-inactivated PRV Be and low-MOI PRV 180 coinfection. (A) N-compartment axons were infected with either PRV 180 at an MOI of 1 (control; top row of photographs) or together with UV-inactivated Be at an MOI of 100 (bottom row of photographs). The axons were also treated with DiO (green) to label cell bodies that project axons to the N compartment. Low-magnification images of cell bodies in the S compartment are shown in the photographs. (B) S-compartment neurons were either infected with 1 MOI of PRV 180 alone or together with UV-PRV Be at an MOI of 100. Images were taken at 48 hpi. Low-magnification images of cell bodies in the S compartment are shown in the photographs. (C) Infectious titers in S compartments at 48 hpi. Axons were infected with PRV 180 at an MOI of 1 alone (control) or together with UV-inactivated Be at an MOI of 100 in the N compartment (+UVBe N) or in the S compartment (+UVBe S) or with the filtered virus stock to test for inhibitors in the medium. (D) N-compartment axons were infected either with PRV 233 at an MOI of 1 alone or together with UV-PRV Be at an MOI of 100 in the N compartment (+UV-Be). Infectious titer values are means plus SEMs (error bars) from three independent experiments. Values that are significantly different by one-sample t test are indicated as follows: **, P < 0.01; ***, P < 0.001, using one sample t test.