TABLE 4 .

Primer and probe sequences developed in this study

PCR assay	PCR conditions	Amplicon size (bp)	Primer/probe	Primer/probe sequencea
Eggerthella-like	56°C annealing, 39 s	84	Egger-like_126F	5′ GACCAACCTGCCTCTTACATT 3′
	72°C extension, 30 s		Egger-like_210R	5′ GCATACATCATGTGATATGTGC 3′
			Egger-like_155-178_pb	5′-FAM-AAAAGAAATTCTGGCTAATACCAA-MGBNFQ-3′
Prevotella buccalis	57°C annealing, 39 s	171	Pbuccalis_455F	5′ GCGCGACGTGTCGTGCA 3′
	72°C extension, 30 s		Pbuccalis_626R	5′ CCGGTTGAGCCGGTACA 3′
			Pbuccalis_587-604_pb	5′-FAM-CGCCAGRTAAGCGTGTTG-MGBNFQ-3′
Prevotella Chemical Formula: timonensis	64°C annealing/ extension, 60 s	90	Ptimonensis_578F	5′ GAGCGTAGGCTGTCTATTAAGC 3′
			Ptimonensis_668R	5′-CTTCCTGCATACTCAAGTCGAC 3′
			Ptimonensis_609-629_pb	5′-FAM-ATTTACCGGCTCAACCGGTGG-MGBNFQ-3′
Prevotella amnii	59°C annealing, 39 s	69	Pamnii_989F	5′ GGCTTGAATTGCAGATGTTTATAT 3′
	72°C extension, 30 s		Pamnii_1058R	5′ CCATGCAGCACCTTCACAAAT 3′
			Pamnii_1014-1033_pb	5′-FAM-AGATGATATATTCCCTTCGG-MGBNFQ-3′

^aFAM, 6-carboxyfluorescein; MGBNFQ, minor groove binder-nonfluorescent quencher.