TABLE 2 .
Mosquito infection rates and oocyst counts from parasites complemented with the episomal D-ssu gene or control plasmid pL0007
| Parasite | Plasmid | No. of transfectionsa | No. of feedingsb | No. of mosquitoes dissected | No. (%) of mosquitoes infected | Avg no. of oocysts (SD)c |
|---|---|---|---|---|---|---|
| By265G(d) | 6 | 148 | 124 (83.8 | 23.0 (20.0) | ||
| pL0007 | 1 | 4 | 73 | 59 (80.8) | 21.5 (20.1) | |
| pL0007_dssuB | 2 | 10 | 224 | 0 (0.0) | 0 | |
| By265 | 6 | 163 | 125 (76.7) | 24.3 (26.3) | ||
| pL0007 | 1 | 3 | 80 | 68 (85.0) | 22.3 (20.2) | |
| pL0007_dssuB | 3, a | 4 | 217 | 0 (0.0) | 0 | |
| b | 2 | 62 | 37 (59.7) | 7.69 (10.1)† | ||
| c | 2 | 49 | 0 (0.0) | 0 | ||
| pL0007_dssuN | 2, a | 1 | 29 | 25 (86.2) | 22.4 (18.0) | |
| b | 2 | 60 | 51 (85.0) | 25.3 (20.6) | ||
| N67 | 5 | 218 | 209 (95.9) | 219.3 (115.9) | ||
| pL0007 | 1 | 4 | 107 | 100 (93.5) | 219.5 (138.3) | |
| pL0007_dssuB | 2, a | 4 | 132 | 123 (93.2) | 205.2 (116.3) | |
| b | 1 | 27 | 24 (88.9) | 212.4 (136.8) | ||
| pL0007_dssuN | 2, a | 2 | 65 | 53 (81.5) | 132.8 (117.4)* | |
| b | 3 | 115 | 60 (52.2) | 70.0 (93.2)† |
a
Number of independent transfections conducted. The letters “a” to “c” indicate individual transfection experiments.
b
Number of independent mosquito feedings.
c
†, P < 0.001, and *, P < 0.05, by t test compared with untransfected controls.