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. 2015 Mar 24;6(2):e00278-15. doi: 10.1128/mBio.00278-15

FIG 3 .

FIG 3 

Comparison of the manganese accumulations and relative gene expression levels of 5448 wild-type GAS, 5448ΔmntE, and 5448ΔmntE::mntE strains. (A) Intracellular manganese analysis of 5448, 5448ΔmntE, and 5448ΔmntE::mntE grown on Todd-Hewitt agar plus 1% yeast extract in the absence (black bars) or presence (white bars) of 1 mM manganese. One-way ANOVA was performed comparing manganese accumulations of 5448 WT and 5448ΔmntE in the presence of manganese (****, P < 0.0001). (B) Gene expression of mntE in 5448 WT, 5448ΔmntE, and 5448ΔmntE::mntE grown in the absence (black bars) or presence (white bars) of 2 mM manganese. Expression of mntE was calculated using the 2−ΔΔCT method using proS as the reference gene. One-way ANOVA was performed comparing expression levels of mntE in 5448 wild type grown in THY broth versus 5448 wild type and 5448ΔmntE::mntE grown in THY broth supplemented with 2 mM manganese (***, P < 0.001). Graphs represent means and standard deviations of three independent experiments.