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. 2015 Apr 7;6(2):e00081-15. doi: 10.1128/mBio.00081-15

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Copyright © 2015 Watanabe et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 5 — Expression and cleavage of wild-type and mutant HA proteins in 293T cells. 293T cells were transfected with Flag-tagged HA expression plasmids. At 24 h posttransfection, cells were harvested and analyzed by Western blotting using anti-Flag antibody. (A) After quantitation of the band intensities by using ImageJ software, the amounts of expression and cleavage for each HA were calculated relative to those for EG/D1 (wt). Each data point is the mean ± SD from three independent experiments. Colors on the x axis highlight the categories of HA mutations as indicated. **, P < 0.01; *, P < 0.05 (Student’s t test). (B) Representative results of Western blotting for EG/D1 and the indicated HA mutants are shown.