FIG 8 .
Neutralizing capacity of human CD4 D1/D2 domain containing immunoadhesins. (A) Schematic representation of six constructs. Human CD4 D1 and D2 domains are shown in green, immunoglobulin (IgG) Fc and constant heavy- and light-chain (CH/CL) regions are shown in black, E51 variable heavy and light (VH/VL) regions are shown in blue, CCR5 mimetic peptides are shown in yellow, and the T-20 fusion inhibitor is shown in red. (B) The ability of human CD4 containing antibody-like constructs (listed on the right) to neutralize a panel of HIV-1, SIVcpz, and SIVgor strains (bottom) is shown. Numbers indicate IC50s (in micrograms per milliliter) from TZM-bl cells, averaged from three different experiments, with a heat map indicating the relative neutralization potency. The highest antibody concentration used was 10 µg/ml. The geometric mean IC50 is shown for each construct. (Only values for SIVcpz and SIVgor strains that were below 10 µg/ml were included in the calculation.)