FIG 2 .

Quantitative PCR of known binding sites of the transcription factors on chromatin. To confirm the success of the ChIP, the sample chromatin from the pBabe (pB)- and CTAR1 (CT1)-expressing cells was subjected to quantitative PCR for known target/binding sites of the transcription factors. For the bcl3 and p50 ChIPs, primer pairs were used to amplify known binding sites within the EGFR promoter, EGFR-pro and KB2. For the pStat3 ChIP, primer pairs were used to amplify known binding sites within the bcl3 gene, the bcl3-promoter and intronic sites HS3 and HS4.