Figure 1.

Identification of miRNAs differentially expressed in normal colorectal mucosae, colorectal adenomas and colorectal cancers. qRT–PCR analysis (TaqMan low-density array A cards, n=377 unique miRNA targets) was used, as described in Materials and Methods, in an initial screen to identify miRNAs expressed in normal mucosae (n=3), colorectal adenomas (n=6) and colorectal cancers (n=3), and to assess interindividual differences in miRNA expression. (A) Representative data summarising the expression of the first 55 miRNAs on the TLDA array are illustrated, with additional data summarised in Supplementary Figure 1. MicroRNA expression is illustrated relative to the expression of the invariant miRNA let-7a. MicroRNAs with consistently increased (n=7; B) or decreased (n=6; C) expression, comparing normal mucosae with colorectal adenomas and cancers, are highlighted.