Figure 5.

Comparison of miR-224 expression in paired primary and metastatic colorectal cancers. qRT–PCR analysis was used, as described in Materials and Methods, to compare miR-224 expression in primary colorectal cancers (n=20) and matched (A) liver and (B) lymph node metastases. MicroRNA-224 expression is illustrated relative to the expression of the invariant miRNA let-7a; all samples were analysed in triplicate, with experimental errors calculated as previously described (Smith et al, 2012). Fold changes in miR-224 expression and associated P-values are summarised for sample pairs where significant differences in gene expression were identified.