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. 2015 Mar 17;112(9):1510–1518. doi: 10.1038/bjc.2015.96

Figure 3.

Figure 3

Surface expression of markers on TILs after overnight or GentleMACS disaggregation. (A) Flow cytometric analysis of CD4, CD8, EpCAM, HLA A,B,C and HLA DR,DP, DQ expression on isolated, uncultured TILs from four renal cell carcinoma biopsies after overnight (black empty line) or GentleMACS (grey-filled line) disaggregation. (B) Effect of MHC class I and class II blockade on the release of IFNγ after 24-h co-culture of TILs with an autologous tumour in two RCC biopsies. **P<0.01 Unpaired t-test.