Figure 3.

TTK knockdown leads to apoptotic and necrotic death of pancreatic cancer cells. Western blot analysis for induction of apoptosis showed enhanced PARP (A) and/or Caspase-3 (B) cleavage 72 h after siRNA transfection in all three pancreatic cancer cell lines used in the experiments. Data are representative of at least three independent experiments. Beta-actin served as loading control. (C) Flow cytometry analyses via Annexin-V/PI staining in PaTu-8988 T cells revealed significantly increased proportions of early (Annexin-V signal only) and late (Annexin-V plus PI signal) apoptotic cells as well as necrotic cells (PI staining only) following transient TTK knockdown. Data represent an average of three experiments. siC=cells transfected with non-silencing control siRNA; NT=untreated cells.