Table 3. The sensitivity and specificity of stool-based miR-18a and miR-221.
| Stool-based microRNA | microRNA-221 | microRNA-18a | ||||
|---|---|---|---|---|---|---|
| Cutoff valuea | 48 (best) | 68 (80% specificity) | 128 (90% specificity) | 446 (best) | 1845 (80% specificity) | 2886 (90% specificity) |
|
Sensitivities, % (95% CI) | ||||||
| CRC | 62 (55–68) | 52 (45–59) | 35 (28–42) | 61 (53–68) | 46 (39–54) | 35 (29–42) |
|
Stage | ||||||
| I+II | 44 (33–53) | 29 (20–40) | 15 (8–26) | 61 (54–71) | 49 (39–59) | 37 (28–27) |
| III+IV | 63 (52–74) | 54 (42–65) | 28 (19–39) | 58 (47–68) | 40 (30–51) | 31 (21–41) |
|
Location | ||||||
| Proximal | 49 (35–63) | 41 (28–58) | 29 (18–44) | 62 (47–75) | 46 (32–61) | 36 (23–51) |
| Distal | 63 (52–74) | 57 (46–69) | 35 (25–47) | 59 (47–69) | 46 (35–58) | 37 (26–48) |
| Rectum | 70 (57–84) | 55 (42–67) | 38 (26–51) | 62 (49–74) | 48 (36–61) | 33 (22–46) |
|
Antibiotic intake | ||||||
| Yes | 65 (44–83) | 58 (37–77) | 33 (26–41) | 62 (55–70) | 48 (40–55) | 35 (17–56) |
| No | 61 (53–68) | 51 (43–59) | 46 (27–67) | 50 (30–70) | 38 (20–59) | 35 (28–43) |
| Specificity, % (95% CI) | 74 (67–80) | 80 (74–86) | 90 (85–94) | 69 (62–75) | 80 (74–86) | 90 (85–94) |
Abbreviations: CI=confidence interval; CRC=colorectal carcinoma; ROC=receiver operating characteristic.
a
Cutoff values were expressed as copies of two microRNAs per nanogram of extracted stool total RNA. The cutoff values were selected using ROC curves for reference.