Figure 2.

Golgi cisternae (rat sialyltransferase transmembrane domain and cytosolic tail fused to the yellow fluorescent protein, red) and the ERES marker (SEC16-GFP, green) visualized in tobacco leaf epidermal cells. Images from time-lapse sequence acquired at the cortical region of tobacco leaf epidermal cell with a Zeiss LSM510 confocal microscope. The Sec16 marker distributes at the peri-Golgi area (arrowheads) as well as to structures of unknown identity that are not associated with the Golgi marker (arrows; Takagi et al., 2013). The structures labeled by Sec16 can assume a ring-like shape (Takagi et al., 2013). Time of frames in the sequence is indicated at the left-hand corner of images (seconds). *, A chloroplast that is visible through chlorophyll autofluorescence. Bars = 5 and 1 μm (inset).