Figure 1.
Expression of MYB96 in seeds. A, MYB96 expression in dry seeds and during seed germination. Wild-type seeds were kept in darkness at 4oC for 72 h (stratification) and transferred to long-day conditions at 22oC for germination. Transcript accumulation was analyzed by qRT-PCR. The EUKARYOTIC TRANSLATION INITIATION FACTOR4A1 (eIF4a) gene (At3g13920) was used as an internal control. Biological triplicates were averaged. Error bars indicate se. DAC, Days after cold stratification. B, Histochemical staining analysis. The pMYB96:GUS transgenic seeds, in which a promoter sequence covering an approximately 2-kb region upstream of the MYB96 transcription start site was transcriptionally fused to a GUS-coding sequence, were subject to GUS staining. Bars = 0.7 μm. C, Induction of MYB96 by ABA in seeds. Stratified wild-type seeds were transferred to MS medium supplemented with 5 μm ABA or GA and incubated for 1 d under long-day conditions. Transcript accumulation was analyzed by qRT-PCR. Biological triplicates were averaged. Error bars indicate se.