Figure 6.

Lipid breakdown in wild-type Columbia-0 (Col-0), myb96-ox, and myb96-1 seeds during seed germination in the presence of ABA. Seeds were germinated on MS plates supplemented with 1 μm ABA and incubated at 23°C under long-day conditions for 3 d. Lipid breakdown was determined by measuring the abundance of total fatty acid (FA; A) and eicosenoic acid (20:1; B). Fatty acid content was expressed as the percentage of the amount present in dry seeds of the indicated genotypes. At least 30 seeds per genotype were measured in each replicate. Biological triplicates were averaged. Error bars indicate se.