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. 2015 Apr 13;168(2):677–689. doi: 10.1104/pp.15.00162

Figure 7.

Figure 7.

Genetic hierarchy of ABI4 and MYB96. A, Germination rate of myb96-ox/abi4-1. The seed germination percentage of each genotype on 1 μm ABA was scored at the indicated time points after cold stratification. At least 50 seeds per genotype were measured in each replicate. Biological triplicates were averaged. Error bars indicate se. B, Lipid breakdown of myb96-ox/abi4-1. Seeds were germinated on MS plates supplemented with 1 μm ABA and incubated at 23°C under long-day conditions for 3 d. Eicosenoic acid (20:1) content was expressed as a percentage of the amount in dry seeds of the indicated genotypes. At least 30 seeds per genotype were measured in each replicate. Biological triplicates were averaged. Error bars indicate se. C, Proposed role of MYB96 during seed germination. The ABA-inducible R2R3-type MYB96 transcription factor regulates ABI4 expression by binding directly to its gene promoter. The MYB96-ABI4 module inhibits lipid mobilization specifically in the embryo, thereby contributing to delaying seed germination under suboptimal conditions. Col-0, Wild-type Columbia-0.