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. 2015 Apr 20;168(2):561–574. doi: 10.1104/pp.15.00321

Figure 2.

Figure 2.

CrDOF expression is influenced by daylength and the circadian clock. A, CrDOF transcript levels in CW15 during a 24-h course in LD (solid line) and SD (dashed line). Data are from three independent experiments ± se. B, Circadian expression of CrDOF in a 24-h LD followed by 48 h in LL (gray) or DD (black). Data are from three independent experiments ± se. C, Immunodetection of CrDOF in a purified recombinant protein fraction (Rec; 1 µg) and algal crude extract (Native; 50 µg). Black lines are MM markers in kD. D, CrDOF protein levels in CW15 in a 24-h course in LD (solid line) and SD (dashed line). Data are from two independent experiments ± se. *, P < 0.05. E, Confocal images of CW15 and CrDOFox:YFP lines. SYTOBlue45 fluorescence around nuclear regions (blue), YFP (yellow), chloroplast (red) are shown. Bars = 5 μm.