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. 2015 Apr 17;168(2):708–720. doi: 10.1104/pp.15.00402

Figure 5.

Figure 5.

KR-substituted IAA1 proteins are degraded rapidly in vivo. A to D, Three independent lines expressing the indicated HIS6x-HA3x-IAA1 proteins were tested in cycloheximide degradation assays. Plants were treated with 200 μg mL−1 cycloheximide or mock (0) for 45 min, and HIS6x-HA3x-IAA1 was visualized by anti-HA immunoblotting. Ponceau S-stained membranes (PSs) are shown for loading controls. Molecular mass markers (in kilodaltons) are indicated to the right. A, Wild-type (Wt) and 2aKR lines. Lanes 1 to 6 and lanes 7 and 8 have 30 and 150 μg of total protein, respectively. B, Wild-type and 2bKR lines. Lanes 1 to 8 have 30 μg of total protein. C, Wild-type and 3aKR lines. Lanes 1 to 4 and 5 to 8 have 30 and 60 μg of total protein, respectively. D, Wild-type and 3bKR lines. All lanes have 30 μg of total protein loaded. E, Wild-type and 6KR lines. All lanes have 80 μg of total protein. F, Wild-type and 8KR lines. All lanes have 80 μg of total protein.