Figure 3.
ixr1 seeds have altered mucilage structure and cellulosic ray density. A, B, E, and F, RR-stained wild-type, cesa5-1, ixr1-1, and ixr1-2 seeds, respectively. Black arrowheads indicate ixr1-1 mucilage rays. Bars = 200 μm. C, D, G, and H, S4B-stained wild-type, cesa5-1, ixr1-1, and ixr1-2 seeds, respectively. White arrowhead indicates columella with primary wall attached. Bars = 10 μm. Arrows indicate rays. I, Ray length of S4B-stained wild-type, cesa5-1, ixr1-1, and ixr1-2 seeds. Error bars indicate se. Single asterisks indicate ray length significantly reduced compared with the wild type, and double asterisks indicate ray length significantly increased compared with the wild type, based on Student’s t test (P < 0.05).