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. 2015 Apr 14;168(2):443–451. doi: 10.1104/pp.15.00503

Figure 3.

Figure 3.

Western analyses of NDH-1L and NDH-1M complexes from the wild-type (WT), ∆ndhQ, and M55 strains. A, Immunodetection of Ndh subunits in thylakoid membranes from the wild-type (including indicated serial dilutions), ∆ndhQ, and M55 mutants. Immunoblotting was performed using antibodies against hydrophilic Ndh subunits (NdhH, NdhI, NdhK, and NdhM). Lanes were loaded with thylakoid membrane proteins corresponding to 1 µg of Chl a. In the lowest lane, ATPβ was used as a loading control. B, Thylakoid protein complexes isolated from the wild type and mutants were separated by blue native (BN)-PAGE. Thylakoid membrane extract corresponding to 9 µg of Chl a was loaded onto each lane. The positions of the NDH-1L and NDH-1M complexes are indicated by red and blue arrows, respectively. C, Protein complexes were electroblotted to a polyvinylidene difluoride membrane, and the membrane was cross reacted with anti-NdhH, NdhI, NdhK, and NdhM to probe the assembly of the NDH-1L and NDH-1M complexes.