Effect of temperature and incubation time on the stability of NDH-1L and NDH-1M complexes from the wild-type (WT) and ∆ndhQ strains. A and D, Profiles of BN-PAGE of the thylakoid membranes isolated from the wild-type and ΔndhQ strains. The membranes were incubated with 1.5% (w/v) DM on ice for 60 min (at 25°C for 0 min) or at 25°C for 10, 20, 30, and 60 min. Western analyses of NDH-1L (red arrows) and NDH-1M (blue arrows) of the wild type (B and C) and ΔndhQ (E and F) isolated in A and D, using antibodies against NdhH and NdhM.