Figure 4.

Effect of NOB1 and miR-646 expression on the cell cycle and apoptosis in renal cancer cells. (A and B) 786-O and ACHN cells were transfected with pre-miR-646, NOB1-shRNA, control percursor or control shRNA, respectively. After being cultured for 48 h, cells were fixed and stained with propidium iodide and cell cycle was analysed by flow cytometry. Representative analysis of three independent experiments is shown. (C and D) Quantities of the apoptosis cells with Annexin V-FITC/PI stain and flow cytometry analysis. miR-646 expression or NOB1 silencing causes G1-phase cell cycle arrest, and induced early apoptosis (Annexin V+/PI−). Three independent experiments were performed in each group (*P<0.05).