Figure 6.

The effect of miR-646 and NOB1-shRNA treatment on tumour growth in 786-O and ACHN cells and xenograft model. (A and B) Soft agar assay was performed to investigate the effect of miR-646 on tumour growth in vitro. 786-O cells had been infected with miR-646 precursor, control precursor, non-treatment or NOB1-shRNA were plated in wells coated with agar. (C and D) The same assay was performed on ACHN cells. Significant differences between transfected cells and mock-infected cells were determined using the two-tailed Student's t-test (*P<0.05). miR-646 overexpression or NOB1 silencing decreased colony formation. (E) Xenograft model was used to examine the effect of miR-646 on tumorigenesis in vivo. Tumour volume was calculated as width² × length × 0.5 (mm³) every 5 days. miR-646 overexpression significantly inhibited tumour formation was observed. (F) Vertical bars indicate mean tumour weight at the end of the experiment. *P<0.05 as compared to the control precursor. (G) Tumour volume was evaluated periodically at the indicated days postinoculation. Data represent mean±s.d. of three independent experiments. All error bars indicated s.e.m. (**P<0.01, *P<0.05).