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Gelatin zymography (A) and western blot analysis (B) of CM from senescent (H357F; BICR18F; H2O2-treated NHOF1) and non-senescent (BICR66F; NHOF1) fibroblasts. The data show gelatinase activity (molecular weight 75 Kd) and active MMP-2 (molecular weight 64 Kd) in CM from senescent but not non-senescent cells.
