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Assembly of the STT3B complex in MI8-5 cells. Digitonin extracts of CHO, MI8-5 cells or MI8-5 cells expressing STT3B-V5H6 were resolved by BN-PAGE and OST complexes detected by protein immunoblotting. The protein blots were aligned using native molecular-weight markers. The brackets marked by an asterisk indicates STT3B complexes with a lower apparent molecular weight than the 550 kDa STT3B complex present in CHO cells.
