Table 1. Data-collection statistics.
Values after a solidus (/) are for the high-resolution shell.
| Lysozyme | |||||
|---|---|---|---|---|---|
| Data | 30min Control | Control 1 | Vacuum Thick | Vacuum Thin | Proteinase K |
| Wavelength () | 1.7712 | 1.7711 | 1.7712 | 1.7711 | 0.9537 |
| Total frames | 360 | 360 | 360 | 360 | 360 |
| Oscillation | 1 | 1 | 1 | 1 | 1 |
| Space group | P43212 | P43212 | P43212 | P43212 | P43212 |
| Unit cell (, ) | a = 78.97 | a = 78.18 | a = 76.77 | a = 76.15 | a = 67.52 |
| b = 78.97 | b = 78.18 | b = 76.77 | b = 76.15 | b = 67.52 | |
| c = 36.91 | c = 37.00 | c = 36.83 | c = 34.33 | c = 102.59 | |
| = 90.00 | = 90.00 | = 90.00 | = 90.00 | = 90.00 | |
| = 90.00 | = 90.00 | = 90.00 | = 90.00 | = 90.00 | |
| = 90.00 | = 90.00 | = 90.00 | = 90.00 | = 90.00 | |
| Resolution () | 1.8 | 1.8 | 1.8 | 2.22 | 1.91 |
| Mosaicity () | 0.062 | 0.189 | 0.281 | 0.283 | 0.195 |
| Total reflections | 286640/37496 | 279557/37193 | 259501/32934 | 135977/20210 | 474867/61496 |
| Unique reflections† | 20574/3265 | 20292/3249 | 19526/3128 | 9542/1507 | 35182/5645 |
| Redundancy | 13.93/11.48 | 13.78/11.45 | 13.29/10.53 | 14.25/13.41 | 13.50/10.89 |
| Completeness (%) | 99.1/97.5 | 99.8/98.8 | 99.7/98.4 | 99.6/97.4 | 99.9/99.5 |
| Mean I/(I) | 24.28/15.42 | 29.57/10.22 | 27.65/13.25 | 20.88/4.07 | 47.16/22.35 |
| R merge (%)‡ | 9.8/11.9 | 6.7/17.7 | 7.7/15.3 | 8.3/64.4 | 5.3/10.6 |
| R meas (%)‡ | 10.2/12.4 | 6.9/18.5 | 8.0/16.0 | 8.6/66.9 | 5.5/11.1 |
| Wilson B | 17.82 | 21.59 | 22.79 | 47.99 | 14.25 |
†
Friedel pairs kept separate.
‡
R merge = hkl i|Ii(hkl) I(hkl)|/hkl iIi(hkl) and R meas (R r.i.m.) = hkl[N/(N 1)]1/2 i|Ii(hkl) I(hkl)|/hkl iIi(hkl), where Ii(hkl) is the ith intensity measurement of reflection hkl, I(hkl) is its average and N is the redundancy of a given reflection.