Figure 6.

Knockdown of cdc42 and sec10 delays retrograde intracellular transport. (A) Dorsal view of the head melanocytes of a 120-hpf dark-adapted embryo (pretreatment). The boxed region encompasses the area of high magnification seen in (B–D). (C) Embryos were treated with epinephrine, and the melanosomes rapidly decreased in size. (D) The endpoint occurred when the pigment granules showed perinuclear localization, and the size was maximally decreased. (E) Quantification of the response time to the endpoint for epinephrine treatment in control and cdc42, sec10, and cdc42+sec10 morphants is shown. *P = 0.0177; **P = 0.0154; ***P = 0.0008. This experiment was repeated three times with similar results.