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. 2015 Jun 3;5:10897. doi: 10.1038/srep10897

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Schematic illustration of the aptamer-based sandwich assay. (a) Sample preparations for the use of the current antibody-based ELISA kit. (b) Aptamer-based sandwich assay platform consisting of the following three major factors: the functionalized surface by capture_aptamer (LCN2_apta4), hybridization, and signal read-out by HRP-labeled reporter_aptamer (LCN2_apta2), which enables the device to be run on straight serum, and there is no need for sample dilution. All parts of this figure were drawn by the authors K-A. L. and J-Y. A.