Fig. 1.

Highly efficient direct conversion of CiNCs from four samples of human fibroblasts by a chemical cocktail of six compounds. (A) Representative morphologies of transdifferentiated cells stained with neuronal marker Tuj1 from the fibroblasts at 3 weeks exposure to the chemical cocktail. (B) Immunofluorescence analysis of CiNCs in Hum22 fibroblasts for staining with Tuj1 antibody. PI, propidium iodide. (C) Quantification of Tuj1⁺ CiNCs in Hum22 fibroblasts. (D) Conversion efficiencies of different passage (P) number of the fibroblasts cultured with a 3-week exposure to the chemical cocktail expressed as neuronal purity.