Fig. 4.

Highly efficient direct conversion of functional CiNCs from four samples of human fibroblasts by a chemical cocktail of six compounds. (A) Three weeks after treatment with the chemical cocktail, Tuj1-positive CiNCs co-expressed vesicular glutamine transporter 1 (vGLUT1). (B) Tuj1-positive CiNCs co-expressed GABAergic neuron marker (GABA) at three weeks after treatment with the chemical cocktail. (C) Co-expression of Tuj1 and tyrosine hydroxylase (TH) in CiNCs from the fibroblasts cultured with a 3-week exposure to the chemical cocktail.