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. 2015 Jun 3;5:10837. doi: 10.1038/srep10837

Figure 1. Characterisation of PsCRN115-transgenic Nicotiana benthamiana.

Figure 1

(a) Western blot analysis of total proteins from leaf extracts of transgenic N. benthamiana lines of T3 generation. Total proteins were extracted from 40-day-old leaf tissues of PsCRN115 transgenic N. benthamiana lines (#13, #18, #27 and #34) and the GFP transgenic N. benthamiana line (GFP). The monoclonal antibodies against GFP were used for protein expression detection. PS is Ponceau stain. (b) Seed germination of N. benthamiana plants on MS medium 12 days after sowing. (c) The post-germination growth of transgenic seedlings on MS medium. The root length (10 seedlings for each transgenic line) was recorded 14 days after sowing. (d) The developmental phenotypes of transgenic plants at 7 (above) and 10 weeks (below) post-germination. WT, wild-type N. benthamiana used as recipient; GFP, GFP-transgenic N. benthamiana; #13, a PsCRN115-transgenic line used as an example.